US 12,188,029 B2
Method for the transient expression of nucleic acids in plants
Pei Su, Research Triangle Park, NC (US); Kasimalai Azhakanandamn, Research Triangle Park, NC (US); Michele Yarnall, Research Triangle Park, NC (US); and Rachel Whinna, Research Triangle Park, NC (US)
Assigned to Syngenta Crop Protection AG, Basel (CH)
Filed by SYNGENTA CROP PROTECTION AG, Basel (CH)
Filed on Dec. 21, 2021, as Appl. No. 17/557,847.
Claims priority of provisional application 63/128,917, filed on Dec. 22, 2020.
Prior Publication US 2022/0195444 A1, Jun. 23, 2022
Int. Cl. C12N 15/82 (2006.01)
CPC C12N 15/8205 (2013.01) 10 Claims
 
1. A method of transiently expressing a nucleotide sequence in a plant part in planta comprising the steps of: a) wounding a plant part; b) Agrobacterium-infiltrating of an expression cassette comprising at least one nucleotide sequence operably linked to a promoter into the plant part in planta, and c) transiently expressing said at least one nucleotide sequence in the plant part, wherein Agrobacterium-infiltrating comprises cutting the plant stem above the second node and pipetting agrobacteria onto the cut site and, wherein the Agrobacteria enter the leafroll and infect a wounded nascent leaf.