	US 12,188,027 B2
	Surface expression vector using two kinds of promoters derived from Lactobacillus casei for concurrently expressing two target proteins and method for expressing proteins on microbial surface by using same
Young Chul Park, Seoul (KR); Dae Eun Ki, Seoul (KR); Gyeong Jun Nam, Yongin-si (KR); Se Eun Byeon, Hwaseong-si (KR); Ha Na Moon, Yongin-si (KR); Hyun Jun Kang, Goyang-si (KR); and Kyung Soo Hahm, Seoul (KR)
Assigned to BIOLEADERS CORPORATION, Yongin-si (KR)
Appl. No. 17/281,756
Filed by BIOLEADERS CORPORATION, Yongin-si (KR)
PCT Filed Oct. 10, 2019, PCT No. PCT/KR2019/013261 § 371(c)(1), (2) Date Mar. 31, 2021, PCT Pub. No. WO2020/076079, PCT Pub. Date Apr. 16, 2020.
Claims priority of application No. 10-2018-0120547 (KR), filed on Oct. 10, 2018.
Prior Publication US 2023/0295644 A1, Sep. 21, 2023
Int. Cl. C12N 15/74 (2006.01); C12N 9/88 (2006.01); C12N 15/62 (2006.01)

CPC C12N 15/746 (2013.01) [C12N 9/88 (2013.01); C12N 15/62 (2013.01); C07K 2319/035 (2013.01); C12Y 401/02013 (2013.01)]

11 Claims

1. A surface expression vector for expressing target proteins, the surface expression vector comprising: a first promoter, a gene encoding a poly-gamma-glutamate synthetase complex for surface anchoring, and a gene encoding the target protein; and a second promoter, a gene encoding a poly-gamma-glutamate synthetase complex for surface anchoring, and a gene encoding a target protein,

wherein the first promoter is represented by SEQ ID NO: 1,

wherein the second promoter is represented by SEQ ID NO: 2,

wherein the gene encoding the poly-gamma-glutamate synthetase complex is pgsA and has the nucleotide sequence of any one of SEQ ID NOs: 21 to 23 and 32 to 34.