US 12,188,026 B2
Surface expression vector using poly-gamma-glutamate synthetic gene derived from strain in Bacillus, and method for expressing protein on surface of microorganism, using same
Young Chul Park, Seoul (KR); Jae Won Jeon, Seoul (KR); Hong Gyu Park, Seoul (KR); Hyun Jun Kang, Goyang-si (KR); Se Eun Byeon, Hwaseong-si (KR); Kwang Seo Park, Goyang-si (KR); and Dae Eun Ki, Seoul (KR)
Assigned to BIOLEADERS CORPORATION, Yongin-si (KR)
Appl. No. 17/253,259
Filed by BIOLEADERS CORPORATION, Yongin-si (KR)
PCT Filed Jun. 24, 2019, PCT No. PCT/KR2019/007592
§ 371(c)(1), (2) Date Dec. 17, 2020,
PCT Pub. No. WO2020/004877, PCT Pub. Date Jan. 2, 2020.
Claims priority of application No. 10-2018-0073345 (KR), filed on Jun. 26, 2018.
Prior Publication US 2022/0090099 A1, Mar. 24, 2022
Int. Cl. C12N 15/74 (2006.01); C12N 9/12 (2006.01)
CPC C12N 15/746 (2013.01) [C12N 9/1288 (2013.01); C12Y 207/08005 (2013.01)] 14 Claims
 
1. A surface expression vector for expression of a target protein, the surface expression vector comprising:
a gene encoding pgsA; and
a gene encoding a target protein,
wherein the gene encoding the pgsA consists of the nucleotide sequence of any one of SEQ ID NOs: 13 to 15 and 24 to 26.