| CPC C12N 15/1075 (2013.01) [C12Q 1/44 (2013.01); C12Q 1/6806 (2013.01)] | 20 Claims |
|
1. A method for identifying a nucleic acid encoding an active catalytic molecule in a plurality of nucleic acids, comprising:
providing a plurality of compartments, each compartment comprising no more than one nucleic acid molecule encoding a catalytic biomolecule,
subjecting the compartments to conditions suitable for synthesis of the catalytic biomolecule,
providing substrate nucleotide to the compartments for said catalytic biomolecule,
wherein the substrate nucleotide cannot be incorporated into synthesized nucleic acid by polymerase,
subjecting said compartments to reaction conditions that allow catalytic molecule enzymatic activity to occur,
wherein said activity results in the modification of the substrate nucleotide, so that the nucleotide becomes suitable for incorporating into synthesized nucleic acid by polymerase,
providing into said compartments nucleic acid synthesis reaction mixture comprising reference nucleotides producing at least one copy of nucleic acid contained in the compartment,
wherein modified substrate nucleotide and reference nucleotide are incorporated into nucleic acid sequence by polymerase concurrently,
determining the ratio between reference nucleotide and modified substrate nucleotide in a newly synthesized nucleic acid,
wherein said ratio is indicative of catalytic activity of the catalytic biomolecule encoded by said nucleic acid.
|