Compositions and methods for improved in vitro assembly of polynucleotides
Gregory Lohman, Cambridge, MA (US); Vladimir Potapov, Auburndale, MA (US); John M. Pryor, Gerogetown, MA (US); Rebecca Kucera, Hamilton, MA (US); Katharina Bilotti, Burlington, MA (US); and Richard D. Morgan, Middleton, MA (US)
Assigned to New England Biolabs, Inc., Ipswich, MA (US)
Filed by New England Biolabs, Inc., Ipswich, MA (US)
Filed on Dec. 17, 2021, as Appl. No. 17/644,987.
Application 17/644,987 is a continuation in part of application No. 17/644,516, filed on Dec. 15, 2021.
Application 17/644,516 is a continuation in part of application No. 17/286,066, previously published as PCT/US2019/056670, filed on Oct. 17, 2019.
Claims priority of provisional application 63/213,859, filed on Jun. 23, 2021.
Claims priority of provisional application 63/213,807, filed on Jun. 23, 2021.
Claims priority of provisional application 63/125,530, filed on Dec. 15, 2020.
Claims priority of provisional application 62/909,641, filed on Oct. 2, 2019.
Claims priority of provisional application 62/820,435, filed on Mar. 19, 2019.
Claims priority of provisional application 62/747,874, filed on Oct. 19, 2018.
Prior Publication US 2022/0177875 A1, Jun. 9, 2022
1. A synthetic self-complementary oligonucleotide that comprises a double-stranded region and a single strand loop, wherein the double-stranded region contains a recognition sequence for PaqCI, has unligatable 3′ and 5′ ends and cannot be cleaved by PaqCI.