	US 12,188,007 B2
	Method of cultivating algae and photobioreactor
Tomohisa Hasunuma, Kobe (JP); Yuichi Kato, Kobe (JP); Yasuo Fujikawa, Yokohama (JP); and Tomohiro Tsurumoto, Yokohama (JP)
Assigned to NICHIA CORPORATION, Anan (JP)
Filed by NICHIA CORPORATION, Anan (JP)
Filed on Sep. 12, 2022, as Appl. No. 17/942,812.
Application 17/942,812 is a division of application No. 16/802,037, filed on Feb. 26, 2020, granted, now 11,473,051.
Claims priority of application No. 2019-034308 (JP), filed on Feb. 27, 2019; and application No. 2020-015613 (JP), filed on Jan. 31, 2020.
Prior Publication US 2023/0002721 A1, Jan. 5, 2023
Int. Cl. C12N 1/12 (2006.01); A01G 33/00 (2006.01); C12M 1/00 (2006.01); C12N 13/00 (2006.01)

CPC C12N 1/12 (2013.01) [C12M 21/02 (2013.01); C12N 13/00 (2013.01); A01G 33/00 (2013.01)]

18 Claims

1. A method of cultivating algal cells of an algae belonging to a class selected from Chlorophyceae, Euglenophyceae, Bacillariophyceae and Haptophyceae, the method comprising:

irradiating the algal cells with an artificial light having a ratio of (i) photon flux density in a wavelength range of 520-630 nm to (ii) photosynthetic photon flux density, that is 65% or more; and

measuring a cell size of the algal cells; and

switching between performing the irradiation and stopping the irradiation of the algal cells with the artificial light, or changing the photon flux density in the wavelength range of 520-630 nm, according to the measured cell size.