	US 12,188,006 B2
	Method for producing highly viable dried microbial cells
Satoshi Miida, Tokyo (JP); Yuzuru Makino, Tokyo (JP); Akihisa Matsui, Tokyo (JP); Masahiko Ito, Tokyo (JP); and Yasuhiro Moteki, Tokyo (JP)
Assigned to KABUSHIKI KAISHA YAKULT HONSHA, Tokyo (JP)
Appl. No. 17/041,749
Filed by KABUSHIKI KAISHA YAKULT HONSHA, Tokyo (JP)
PCT Filed Feb. 14, 2019, PCT No. PCT/JP2019/005204 § 371(c)(1), (2) Date Sep. 25, 2020, PCT Pub. No. WO2019/193841, PCT Pub. Date Oct. 10, 2019.
Claims priority of application No. 2018-072833 (JP), filed on Apr. 5, 2018.
Prior Publication US 2021/0024878 A1, Jan. 28, 2021
Int. Cl. C12N 1/04 (2006.01); C12N 1/20 (2006.01); C12R 1/225 (2006.01)

CPC C12N 1/04 (2013.01) [C12N 1/20 (2013.01); C12N 1/205 (2021.05); C12N 2523/00 (2013.01); C12R 2001/225 (2021.05)]

2 Claims

1. A method for improving viability of Lactobacillus casei cells dried by lyophilization, the method consisting of:

heating the dried cells at 35 to 37° C. for 1 to 2 days prior to storage; or

cooling the dried cells at 2° C. for 1 day, and then heating the dried cells at 35 to 37° C. for 1 to 2 days prior to storage,

wherein the storage is at 22° C. or lower.