US 12,187,998 B2
Culture container, method for manufacturing culture container, laminated structure, and method for manufacturing laminated structure
Takahiko Totani, Kanagawa (JP); Satoshi Tanaka, Kanagawa (JP); and Yosuke Matsuoka, Kanagawa (JP)
Assigned to TOYO SEIKAN GROUP HOLDINGS, LTD., Tokyo (JP)
Filed by TOYO SEIKAN GROUP HOLDINGS, LTD., Tokyo (JP)
Filed on Jan. 15, 2020, as Appl. No. 16/743,911.
Application 16/743,911 is a continuation of application No. PCT/JP2018/025017, filed on Jul. 2, 2018.
Claims priority of application No. 2017-142318 (JP), filed on Jul. 22, 2017; and application No. 2017-201441 (JP), filed on Oct. 17, 2017.
Prior Publication US 2020/0148992 A1, May 14, 2020
Int. Cl. C12M 1/00 (2006.01); C12M 1/12 (2006.01)
CPC C12M 23/26 (2013.01) [C12M 23/20 (2013.01); C12M 25/02 (2013.01)] 3 Claims
OG exemplary drawing
 
1. A culture container made of a flexible packaging material, the culture container comprising:
a first side surface portion and a second side surface portion that are mutually opposed, wherein at least an inner surface side of the first side surface portion has a surface treatment such that, when a contact angle hysteresis is defined as a difference between an advance contact angle (θa) and a retreat contact angle (θr) at a time when water slips such that the contact angle hysteresis is equal to (θa−θr), a contact angle hysteresis of the surface on the inner surface side of the first side surface portion becomes larger than a contact angle hysteresis of a surface on an inner surface side of the second side surface portion, and a membrane made of a cell adhesion inhibitor is formed on the inner surface side of the first side surface portion,
wherein at least the inner surface side of the first side surface portion has a hydrophilic treatment,
wherein a base material of the inner surface side of the first side surface portion and a base material of the inner surface side of the second side surface portion consist of polyethylene, and
wherein the membrane made of a cell adhesion inhibitor is not formed on the inner surface side of the second side surface portion.