US 12,187,995 B2
Method and apparatus for producing container, cell culture vessel, method for culturing cells, method for producing cell culture vessel, and apparatus for producing cell culture vessel
Ryo Suenaga, Yokohama (JP); Satoshi Tanaka, Yokohama (JP); Takahiko Totani, Yokohama (JP); and Naoki Takahashi, Yokohama (JP)
Assigned to TOYO SEIKAN GROUP HOLDINGS CO., LTD., Tokyo (JP)
Filed by TOYO SEIKAN GROUP HOLDINGS CO., LTD., Tokyo (JP)
Filed on Jun. 13, 2022, as Appl. No. 17/839,142.
Application 17/839,142 is a division of application No. 16/322,012, abandoned, previously published as PCT/JP2017/027229, filed on Jul. 27, 2017.
Claims priority of application No. 2016-152493 (JP), filed on Aug. 3, 2016; and application No. 2016-153701 (JP), filed on Aug. 4, 2016.
Prior Publication US 2022/0306976 A1, Sep. 29, 2022
Int. Cl. C12M 1/00 (2006.01); B29C 35/00 (2006.01); B29C 59/02 (2006.01); B65B 61/24 (2006.01); C12M 1/32 (2006.01); C12M 3/00 (2006.01)
CPC C12M 23/14 (2013.01) [B29C 35/007 (2013.01); B29C 59/02 (2013.01); B65B 61/24 (2013.01); C12M 1/00 (2013.01); C12M 3/00 (2013.01); C12M 23/02 (2013.01); C12M 23/12 (2013.01); C12M 23/58 (2013.01); C12M 47/04 (2013.01)] 10 Claims
OG exemplary drawing
 
1. A cell culture vessel comprising:
a first vessel wall as a bottom wall, the first vessel wall having gas permeability;
a second vessel wall disposed in contact with a peripheral edge portion of the first vessel wall, and having a bulge shape protruding relative to the first vessel wall radially inward of the peripheral edge portion; and
a charge/discharge port communicating to a culture space surrounded by the first vessel wall and the bulge shape of the second vessel wall,
wherein the first vessel wall comprises a central section radially inward of the peripheral edge portion,
wherein the entirety of the first vessel wall is flat and the central section of the first vessel wall has a flat film shape so as to form a flat bottom culture surface in the culture space, and
wherein the charge/discharge port has a contact surface where the charge/discharge port is in contact with the first vessel wall, and the contact surface is flush with an inner surface of the first vessel wall.