US 12,187,784 B2
Processes for making a cellular fibronectin composition
Spencer Alford, Newark, CA (US); and Audrey Smith, Menlo Park, CA (US)
Assigned to COMBANGIO, INC., Menlo Park, CA (US)
Filed by COMBANGIO, INC., Menlo Park, CA (US)
Filed on Jul. 1, 2022, as Appl. No. 17/856,944.
Claims priority of provisional application 63/235,605, filed on Aug. 20, 2021.
Claims priority of provisional application 63/217,952, filed on Jul. 2, 2021.
Prior Publication US 2023/0000954 A1, Jan. 5, 2023
Int. Cl. C07K 14/78 (2006.01); A61K 9/16 (2006.01); A61K 35/28 (2015.01); A61K 38/17 (2006.01); A61K 38/18 (2006.01); A61K 38/36 (2006.01); A61K 38/39 (2006.01); A61K 45/06 (2006.01); A61K 47/02 (2006.01); A61K 47/26 (2006.01); A61P 27/02 (2006.01); C07K 14/475 (2006.01); C12N 5/0775 (2010.01)
CPC C07K 14/78 (2013.01) [A61K 9/1623 (2013.01); A61K 35/28 (2013.01); A61K 38/1709 (2013.01); A61K 38/177 (2013.01); A61K 38/1833 (2013.01); A61K 38/1858 (2013.01); A61K 38/1866 (2013.01); A61K 38/36 (2013.01); A61K 38/39 (2013.01); A61K 45/06 (2013.01); A61K 47/02 (2013.01); A61K 47/26 (2013.01); A61P 27/02 (2018.01); C07K 14/475 (2013.01); C12N 5/0663 (2013.01); C12N 5/0668 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A method of making a composition comprising mesenchymal stem cell (MSC)-derived fibronectin (FN), comprising:
(a) culturing mesenchymal stem cells (MSCs) in a cell culture medium, thereby generating conditioned medium that comprises factors comprising MSC-derived FN secreted by the MSCs;
(b) harvesting the conditioned medium thereby producing harvested conditioned medium; and
(c) filtering harvested conditioned medium to produce processed conditioned medium;
wherein the MSCs are bone marrow-derived.