	US 12,187,769 B2
	Cell
Martin Pulé, London (GB); Shaun Cordoba, London (GB); Simon Thomas, London (GB); Shimobi Onuoha, London (GB); and Matteo Righi, London (GB)
Assigned to AUTOLUS LIMITED, London (GB)
Filed by AUTOLUS LIMITED, London (GB)
Filed on May 24, 2023, as Appl. No. 18/323,090.
Application 18/323,090 is a division of application No. 16/646,906, granted, now 11,701,386, issued on Jul. 18, 2023, previously published as PCT/GB2018/052583, filed on Sep. 12, 2018.
Claims priority of application No. 1714718 (GB), filed on Sep. 13, 2017.
Prior Publication US 2024/0075066 A1, Mar. 7, 2024
Int. Cl. C12N 15/62 (2006.01); A61K 35/17 (2015.01); A61K 39/00 (2006.01); C07K 14/47 (2006.01); C07K 14/705 (2006.01); C12N 5/0783 (2010.01)

CPC C07K 14/4705 (2013.01) [A61K 39/4611 (2023.05); A61K 39/4631 (2023.05); A61K 39/464404 (2023.05); A61K 39/464412 (2023.05); C07K 14/705 (2013.01); C12N 5/0637 (2013.01); C12N 15/62 (2013.01); A61K 2239/31 (2023.05); A61K 2239/47 (2023.05); C07K 2319/02 (2013.01); C07K 2319/20 (2013.01); C07K 2319/50 (2013.01); C07K 2319/70 (2013.01)]

5 Claims

1. A cytolytic immune cell which comprises a CAR and an inducible Signal Transducer and Activator of Transcription (STAT) molecule,

wherein

(a) the STAT molecule comprises a first polypeptide comprising a first dimerizing domain (DD) and a second polypeptide comprising a second DD, which second DD specifically binds to the first DD; and wherein the presence of an agent causes dimerization of the first and second DD and induces activation of the STAT molecule, or

(b) the STAT molecule comprises a first polypeptide comprising a first dimerizing domain (DD) and a second polypeptide comprising a second DD, which second DD specifically binds to the first DD; and wherein the presence of an agent causes dissociation of the first and second DD and induces non-activation of the STAT molecule.