US 12,187,765 B2
Preparation of conserved homology 1 domains complexed to ligands
Tatyana I. Igumenova, College Station, TX (US); and Sachin S. Katti, College Station, TX (US)
Filed by Tatyana I. Igumenova, College Station, TX (US); and Sachin S. Katti, College Station, TX (US)
Filed on Aug. 31, 2022, as Appl. No. 17/900,740.
Claims priority of provisional application 63/239,986, filed on Sep. 2, 2021.
Prior Publication US 2023/0063427 A1, Mar. 2, 2023
Int. Cl. C30B 29/58 (2006.01); C07K 1/30 (2006.01); C30B 7/00 (2006.01)
CPC C07K 1/306 (2013.01) [C30B 7/00 (2013.01); C30B 29/58 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A method, comprising:
preparing an aqueous solution including a protein, a ligand, and a membrane mimic, the protein comprising a conserved homology 1 (C1) domain, the ligand being a ligand of the C1 domain, the membrane mimic being selected to facilitate formation of a complex of the C1 domain and the ligand; and
placing the aqueous solution in a protein-crystallization arrangement configured to form a crystal including the complex of the protein and the ligand.