US 12,187,763 B2
Chromatography resin and uses thereof
Hing C. Wong, Miramar, FL (US); Jin-an Jiao, Miramar, FL (US); and Caitlin Prendes, Miramar, FL (US)
Assigned to ImmunityBio, Inc., San Diego, CA (US)
Filed by ImmunityBio, Inc., San Diego, CA (US)
Filed on Feb. 11, 2021, as Appl. No. 17/173,767.
Claims priority of provisional application 62/982,002, filed on Feb. 26, 2020.
Claims priority of provisional application 62/975,141, filed on Feb. 11, 2020.
Prior Publication US 2021/0277054 A1, Sep. 9, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. C07K 1/22 (2006.01); B01D 15/38 (2006.01); B01D 61/02 (2006.01); B01D 61/14 (2006.01); B01D 61/58 (2006.01); C07K 14/54 (2006.01); C07K 14/715 (2006.01); C07K 14/745 (2006.01); C07K 16/36 (2006.01)
CPC C07K 1/22 (2013.01) [B01D 15/3809 (2013.01); B01D 61/027 (2013.01); B01D 61/145 (2013.01); B01D 61/58 (2013.01); C07K 14/5443 (2013.01); C07K 14/7155 (2013.01); C07K 14/745 (2013.01); C07K 16/36 (2013.01); B01D 2315/16 (2013.01); C07K 2317/565 (2013.01); C07K 2319/00 (2013.01)] 30 Claims
 
1. A method of purifying a multi-chain chimeric polypeptide, the method comprising:
loading an affinity chromatography resin with a liquid comprising the multi-chain chimeric polypeptide;
washing the affinity chromatography resin using one or more wash buffer(s); and
eluting the multi-chain chimeric polypeptide using an elution buffer, wherein:
the affinity chromatography resin comprises an anti-tissue factor antibody or antigen-binding fragment thereof comprising a heavy chain variable domain comprising a CDR1 of SEQ ID NO: 1, a CDR2 of SEQ ID NO: 2 or 9, and a CDR3 of SEQ ID NO: 3, and a light chain variable domain comprising a CDR1 of SEQ ID NO: 4, a CDR2 of SEQ ID NO: 5, and a CDR3 of SEQ ID NO: 6, attached to a base resin;
the multi-chain chimeric polypeptide comprises:
(a) a first chimeric polypeptide comprising:
(i) a first target-binding domain comprising a sequence that is at least 80% identical to amino acids 19 to 305 of SEQ ID NO: 70;
(ii) a soluble tissue factor domain comprising a sequence that is at least 80% identical to SEQ ID NO: 10; and
(iii) a first domain of a pair of affinity domains comprising a sequence that is at least 80% identical to SEQ ID NO: 125; and
(b) a second chimeric polypeptide comprising:
(i) a second domain of a pair of affinity domains comprising a sequence that is at least 80% identical to SEQ ID NO: 123; and
(ii) a second target-binding domain comprising a sequence that is at least 80% identical to amino acids 19 to 305 of SEQ ID NO: 70;
the first chimeric polypeptide and the second chimeric polypeptide associate through the binding of the first domain and the second domain of the pair of affinity domains; and
the multi-chain chimeric polypeptide does not stimulate blood coagulation in a mammal.