US 12,187,760 B2
Photon generating substrates for oligonucleotide synthesis
Bichlien Hoang Nguyen, Seattle, WA (US); Karin Strauss, Seattle, WA (US); Jake Allen Smith, Seattle, WA (US); Richard Prescott Rouse, Redmond, WA (US); Douglas Mitchell Carmean, Seattle, WA (US); Matthew David Turner, Carnation, WA (US); and Gagan Gupta, Redmond, WA (US)
Assigned to MICROSOFT TECHNOLOGY LICENSING, LLC, Redmond, WA (US)
Filed by MICROSOFT TECHNOLOGY LICENSING, LLC, Redmond, WA (US)
Filed on May 26, 2021, as Appl. No. 17/331,321.
Prior Publication US 2022/0380403 A1, Dec. 1, 2022
Int. Cl. C07H 21/04 (2006.01); B01J 19/00 (2006.01); B01J 19/12 (2006.01)
CPC C07H 21/04 (2013.01) [B01J 19/0046 (2013.01); B01J 19/121 (2013.01); B01J 19/123 (2013.01); B01J 2219/00527 (2013.01); B01J 2219/00711 (2013.01); B01J 2219/00722 (2013.01); B01J 2219/0879 (2013.01); B01J 2219/1203 (2013.01)] 20 Claims
OG exemplary drawing
 
1. An oligonucleotide synthesis system comprising:
an oligonucleotide synthesizer, configured to perform automated solid-phase synthesis of oligonucleotides and store individual species of nucleotides, the oligonucleotide synthesizer comprising;
a photon generating substrate;
a reaction chamber; and
a solid-state stack comprising:
an optically transparent, solid substrate coated with a plurality of oligonucleotides;
a light source configured to emit a pattern of light at a specified wavelength;
a focusing layer, separate from and adjacent to the solid substrate, configured to direct light from the light source onto the solid substrate, wherein the focusing layer comprises an array of collimating microlenses; and
a circuitry layer configured to control the pattern of light emitted from the light source and incident upon the solid substrate; and
a computing device comprising an oligonucleotide synthesizer control module, connected to the oligonucleotide synthesizer, wherein the oligonucleotide control module is configured to cause the oligonucleotide synthesizer to synthesize the oligonucleotides.