US 12,186,755 B2
Multi-well separation apparatus and reagent delivery device
Ning Chen, Richmond, CA (US); Robert Keith Shanahan, Carlsbad, CA (US); Dayu Teng, San Diego, CA (US); and Daniel Joseph Braun, San Diego, CA (US)
Assigned to DrugArray, Inc., La Jolla, CA (US)
Filed by DrugArray, Inc., La Jolla, CA (US)
Filed on May 28, 2021, as Appl. No. 17/334,544.
Application 17/334,544 is a division of application No. 15/120,057, granted, now 11,090,654, previously published as PCT/US2015/016435, filed on Feb. 18, 2015.
Claims priority of provisional application 61/941,368, filed on Feb. 18, 2014.
Prior Publication US 2021/0283598 A1, Sep. 16, 2021
Int. Cl. B01L 3/00 (2006.01); B01F 31/441 (2022.01); B01F 31/85 (2022.01); G01N 33/50 (2006.01); B01F 101/23 (2022.01)
CPC B01L 3/5085 (2013.01) [B01F 31/441 (2022.01); B01F 31/85 (2022.01); B01L 3/527 (2013.01); G01N 33/5008 (2013.01); B01F 2101/23 (2022.01); B01L 2200/025 (2013.01); B01L 2200/028 (2013.01); B01L 2200/0689 (2013.01); B01L 2200/16 (2013.01); B01L 2300/0609 (2013.01); B01L 2300/0829 (2013.01); B01L 2300/0851 (2013.01); B01L 2400/0433 (2013.01); G01N 2500/10 (2013.01)] 18 Claims
OG exemplary drawing
 
1. A chemical or biological assay device, comprising:
a substrate;
a separation seal; and
a removable separation well structure,
wherein the removable separation well structure comprises a plurality of walls defining a plurality of openings,
wherein the separation seal forms a leak-proof seal between a proximal surface of the substrate and a distal surface of the removable separation well structure, and
wherein the substrate is coupled to the removable separation well structure via the separation seal to form a plurality of separation wells such that each separation well is isolated from other separation wells.
 
6. A method of performing a chemical or biological assay using the device of claim 1, comprising:
i) applying a target agent to the substrate;
ii) coupling the removable separation well structure to the substrate to form the plurality of separation wells, thereby dividing the target agent into a plurality of subpopulations, each subpopulation in one of the plurality of separation wells; and
iii) applying a plurality of test agents to the plurality of subpopulations,
wherein the effects of the plurality of test agents on the target agent are analyzed.