US 12,186,754 B2
MALDI-TOF analysis plate with paper support and use thereof
Patrick Broyer, Saint Cassien (FR); Nadine Perrot, Montluel (FR); Jérôme Blaze, La Terrasse (FR); and Jean-Marie Baumlin, Anzin Saint Aubin (FR)
Assigned to BIOMÉRIEUX, Marcy l'Etoile (FR)
Appl. No. 16/603,687
Filed by BIOMÉRIEUX, Marcy l'Etoile (FR)
PCT Filed Apr. 26, 2018, PCT No. PCT/FR2018/051056
§ 371(c)(1), (2) Date Oct. 8, 2019,
PCT Pub. No. WO2018/197814, PCT Pub. Date Nov. 1, 2018.
Claims priority of application No. 1753705 (FR), filed on Apr. 27, 2017.
Prior Publication US 2020/0114346 A1, Apr. 16, 2020
Int. Cl. B01L 3/00 (2006.01); B01L 9/00 (2006.01); H01J 49/04 (2006.01); H01J 49/40 (2006.01)
CPC B01L 3/508 (2013.01) [B01L 9/50 (2013.01); H01J 49/0418 (2013.01); H01J 49/40 (2013.01); B01L 2300/06 (2013.01); B01L 2300/0816 (2013.01); B01L 2300/126 (2013.01); B01L 2300/161 (2013.01)] 24 Claims
OG exemplary drawing
 
1. A MALDI-TOF analysis plate for the analysis of a biological sample, comprising:
a planar support comprising a top face and a bottom face wherein the planar support comprises at least one sheet of paper material having cellulose fibers; and
at least one ply of a metal material disposed on the top face of the planar support, wherein: the at least one ply of metal material extends over an entirety of the top face of the planar support;
the at least one ply of the metal material defines at least one test face including at least one analysis zone on the at least one analysis zone has a roughness of less than 750 mL/min;
a mechanical deformation is formed in the ply of metal material and the at least one sheet of paper material, and the mechanical deformation outlines the at least one analysis zone or forms a barrier that encloses the at least one analysis zone so as to prevent the biological sample from leaving the analysis zone after the biological sample is deposited; and
the at least one analysis zone enables the biological sample, when deposited on the at least one analysis zone, to be analyzed by mass spectrometry using a MALDI-TOF technique.