| CPC B01L 3/502776 (2013.01) [G01N 21/78 (2013.01); G01N 33/6839 (2013.01); G01N 33/70 (2013.01); B01L 2300/0867 (2013.01); B01L 2300/0883 (2013.01); B01L 2400/0457 (2013.01); G01N 2333/76 (2013.01); G01N 2800/347 (2013.01)] | 22 Claims |
|
1. A method for mixing two reagents to produce a detectable reaction in a microfluidic chip comprising:
providing a microfluidic chip comprising:
a first reagent inlet at a top surface of the microfluidic chip in fluid communication with a first reagent channel, said first reagent channel having a height, a width and a length, said first reagent channel having a first reagent flow rate defined by the height, the width and the length of the first reagent channel;
a second reagent inlet at a top surface of the microfluidic chip in fluid communication with a second reagent channel, said second reagent channel having a height, a width and a length, said second reaction channel having a second reagent flow rate defined by the height, the width and the length of the second reagent channel;
said first reagent channel and said second reagent channel meeting at a junction point, said junction point in fluid communication with a reaction channel, and said reaction channel in fluid communication with an outlet;
applying a quantity of a first reagent solution to the first reagent inlet;
applying a quantity of a second reagent solution to the second reagent inlet;
applying a drop of oil covering and connecting both the first reagent inlet and the second reagent inlet, said oil having a density slightly lower than a density of the first reagent solution and a density slightly lower than a density of the second reagent solution, wherein said oil floats on top of the first reagent solution in the first reagent inlet and the second reagent solution in the second reagent inlet, thereby balancing a first pressure of the first reagent inlet with a second pressure of the second reagent inlet such that a mixing ratio of the first reagent solution and the second reagent solution is determined based on the length of the first reagent channel and the length of the second reagent channel;
said first reagent solution flowing along the first reagent channel at the first reagent channel flow rate and said second reagent solution flowing along the second reagent channel at the second reagent channel flow rate and mixing at the junction point, thereby producing a detectable reaction; and detecting the detectable reaction within the reaction channel.
|