US 12,186,750 B2
Slipchip device for on-chip dilution and size-based extraction of protein labeling reagents
Rebecca Rose Pompano, Charlottesville, VA (US); and Andrew Kinman, Franklin, IN (US)
Assigned to University of Virginia Patent Foundation, Charlottesville, VA (US)
Appl. No. 16/975,017
Filed by University of Virginia Patent Foundation, Charlottesville, VA (US)
PCT Filed Feb. 25, 2019, PCT No. PCT/US2019/019406
§ 371(c)(1), (2) Date Aug. 21, 2020,
PCT Pub. No. WO2019/165363, PCT Pub. Date Aug. 29, 2019.
Claims priority of provisional application 62/634,565, filed on Feb. 23, 2018.
Prior Publication US 2021/0031187 A1, Feb. 4, 2021
Int. Cl. B01L 3/00 (2006.01)
CPC B01L 3/50273 (2013.01) [B01L 2200/025 (2013.01); B01L 2300/045 (2013.01); B01L 2300/047 (2013.01); B01L 2300/0681 (2013.01); B01L 2300/0816 (2013.01); B01L 2300/087 (2013.01); B01L 2400/0475 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A device for carrying out a reaction, the device comprising:
a first part having a first surface and a second part having a second surface opposed to the first surface;
a plurality of first reservoirs located along a portion of the first surface, each of the plurality of first reservoirs configured to maintain at least one first substance;
a plurality of second reservoirs located along a portion of the second surface, each of the plurality of second reservoirs configured to maintain at least one second substance;
a plurality of third reservoirs located along a portion of the second surface, each of the plurality of third reservoirs configured to maintain at least one third substance; and
a plurality of fourth reservoirs located along a portion of the first surface, each of the plurality of fourth reservoirs configured to maintain at least one fourth substance, optionally wherein one or more, further optionally each of the one or more, of the plurality of fourth reservoirs is separated into two, three, four, five, six, or more sub-reservoirs;
wherein the first surface and the second surface are configured to move relative to each other between:
a first position in which the plurality of first, second, third, and fourth reservoirs are not exposed to any of the other first, second, third, or fourth reservoirs;
a second position in which at least one of the plurality of first reservoirs is exposed to at least one of the plurality of the second reservoirs and none of the third or fourth reservoirs;
a third position in which at least one of the plurality of first reservoirs is exposed to at least one of the plurality of the third reservoirs and none of the second or fourth reservoirs;
a fourth position in which at least one of the plurality of fourth reservoirs is exposed to at least one of the plurality of the second or third reservoirs; and
a fifth position in which the at least one of the third reservoirs is positioned with respect to a detection device such that the detection device can pass a detection light beam through the at least one third reservoir in order to assay the contents of the at least one third reservoir,
and wherein the first part and the second part are engaged with each other before and after the relative motion.