| CPC B01L 3/502715 (2013.01) [B01L 7/52 (2013.01); C12Q 1/6844 (2013.01); G01N 1/2035 (2013.01); B01L 2200/04 (2013.01); B01L 2200/16 (2013.01); B01L 2300/0663 (2013.01); B01L 2300/1827 (2013.01); C12N 9/22 (2013.01); C12N 2310/20 (2017.05); G01N 2001/205 (2013.01)] | 16 Claims |
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1. A cartridge for testing a biological sample, the cartridge comprising:
an aliquot staging well;
an aliquot staging well channel in fluid communication with the aliquot staging well;
a first reaction well;
a first reaction well channel in fluid communication with the first reaction well;
a second reaction well;
a second reaction well channel in fluid communication with the second reaction well;
an actuation port configured for coupling with a pump assembly of an analyzer device configured for operating the cartridge; and
a fluid channel control valve assembly that is reconfigurable between a first fluid channel configuration and a second fluid channel configuration; wherein in the first fluid channel configuration the aliquot staging well channel and the first reaction well channel are in fluid communication through the fluid channel control valve assembly for separately directing a first aliquot of a lysis buffer sample solution from the aliquot staging well to the first reaction well via injection of air into the cartridge through the actuation port by the pump assembly of the analyzer device and the second reaction well channel is fluidly isolated from the aliquot staging well channel via the fluid channel control valve assembly to block flow of any of the first aliquot of the lysis buffer sample solution from being transferred to the second reaction well, and wherein in the second fluid channel configuration the aliquot staging well channel and the second reaction well channel are in fluid communication through the fluid channel control valve assembly for separately directing a second aliquot of the lysis buffer sample solution from the aliquot staging well to the second reaction well via injection of air into the cartridge through the actuation port by the pump assembly of the analyzer device and the first reaction well channel is fluidly isolated from the aliquot staging well channel via the fluid channel control valve assembly to block flow of any of the second aliquot of the lysis buffer sample solution from being transferred to the first reaction well.
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