US 12,186,457 B2
Sphingolipid coatings and process for manufacturing sphingolipid coatings effective for inhibiting biofilm formation
Aaron Seitz, Cincinnati, OH (US); Michael J. Edwards, Cincinnati, OH (US); and Erich Gulbins, Cincinnati, OH (US)
Assigned to University of Cincinnati, Cincinnati, OH (US)
Appl. No. 16/060,455
Filed by UNIVERSITY OF CINCINNATI, Cincinnati, OH (US)
PCT Filed Dec. 9, 2016, PCT No. PCT/US2016/065838
§ 371(c)(1), (2) Date Jun. 8, 2018,
PCT Pub. No. WO2017/100580, PCT Pub. Date Jun. 15, 2017.
Claims priority of provisional application 62/265,659, filed on Dec. 10, 2015.
Claims priority of provisional application 62/382,317, filed on Sep. 1, 2016.
Prior Publication US 2018/0353656 A1, Dec. 13, 2018
Int. Cl. A61L 29/16 (2006.01); A61M 16/04 (2006.01); A61M 25/00 (2006.01)
CPC A61L 29/16 (2013.01) [A61M 16/04 (2013.01); A61L 2300/22 (2013.01); A61L 2300/404 (2013.01); A61M 25/0009 (2013.01); A61M 25/0017 (2013.01); A61M 25/0045 (2013.01); A61M 2205/0205 (2013.01); A61M 2205/0238 (2013.01); A61M 2207/00 (2013.01)] 19 Claims
 
1. A method for applying a three dimensional thin film sphingolipid aggregate coating to a surface of a substrate, the method comprising:
a) providing a suspension consisting of an amount of sphingolipid suspended in a preheated medium-to-fast-evaporating organic solvent, wherein the sphingolipid is selected from one or more of sphingosine, phytosphingosine, and psychosine;
b) applying energy to the suspension sufficient to create a colloidal dispersion of sphingolipid in the solvent, wherein applying energy comprises heating the suspension to a temperature within 10° C. of a boiling point of the medium-to-fast-evaporating solvent and sonicating the suspension at between about 20 kHz to about 40 kHz, wherein the heat and energy are applied until aggregates are not visible and the dispersion becomes a solution;
c) coating the surface of the substrate with at least one application of the solution, each application followed directly by a solvent evaporation period, wherein coating comprises immersing the surface in the solution for 1 s and withdrawal from the solution at a rate of at least 1 cm/second,
wherein the method deposits the sphingolipid in aggregates on the substrate surface to provide a three dimensional thin film coating that resists bacterial adhesion and biofilm formation, and wherein the three dimensional thin film coating is less than 10 μm thick.