	US 12,186,454 B2
	Scaffolds fabricated from electrospun decellularized extracellular matrix
Marcelle Machluf, Haifa (IL); Eyal Zussman, Haifa (IL); and Limor Baruch, Haifa (IL)
Assigned to Technion Research & Development Foundation Limited, Haifa (IL)
Filed by Technion Research & Development Foundation Limited, Haifa (IL)
Filed on Jan. 23, 2023, as Appl. No. 18/100,000.
Application 18/100,000 is a division of application No. 16/092,787, abandoned, previously published as PCT/IL2017/050428, filed on Apr. 6, 2017.
Claims priority of application No. 245059 (IL), filed on Apr. 12, 2016.
Prior Publication US 2023/0158208 A1, May 25, 2023
Int. Cl. A61L 27/38 (2006.01); A61K 35/28 (2015.01); A61L 27/18 (2006.01); A61L 27/24 (2006.01); A61L 27/36 (2006.01); A61P 3/10 (2006.01); A61P 9/00 (2006.01)

CPC A61L 27/3804 (2013.01) [A61K 35/28 (2013.01); A61L 27/18 (2013.01); A61L 27/24 (2013.01); A61L 27/3633 (2013.01); A61L 27/38 (2013.01); A61P 3/10 (2018.01); A61P 9/00 (2018.01)]

13 Claims

1. A method of generating a scaffold comprising:

(a) homogenizing decellularized extracellular matrix (ECM) in an organic solvent using a bead homogenizer to generate a homogenate of decellularized ECM;

(b) contacting said homogenate of decellularized ECM with a synthetic polymer so as to increase viscoelasticity of said homogenate of decellularized ECM, wherein an amount of said synthetic polymer in said homogenate of decellularized ECM is between 0.05-1% mass of said homogenate of decellularized ECM; and subsequently

(c) electrospinning said homogenate of decellularized ECM onto a solid surface thereby generating the scaffold composed of ECM fibers; and subsequently

(d) removing said polymer following said electrospinning such that the scaffold is devoid of said synthetic polymer, wherein the method is performed in-vitro.