US 12,186,434 B1
Surfactin-based lipopeptide for mRNA delivery
Mohammed Saeed Alqahtani, Riyadh (SA)
Assigned to KING SAUD UNIVERSITY, Riyadh (SA)
Filed by KING SAUD UNIVERSITY, Riyadh (SA)
Filed on May 13, 2024, as Appl. No. 18/662,099.
This patent is subject to a terminal disclaimer.
Int. Cl. A61K 9/51 (2006.01); C12N 15/88 (2006.01)
CPC A61K 9/5123 (2013.01) [A61K 9/5169 (2013.01); A61K 9/5192 (2013.01); C12N 15/88 (2013.01)] 4 Claims
OG exemplary drawing
 
1. A method of making a lipid nanoparticle platform for mRNA delivery comprising:
providing OleSurf lipids to form an organic phase;
mixing a mRNA in 100 mM citrate buffer at pH 4 to form an aqueous phase; and
mixing one part of the organic phase with three parts of the aqueous phase to obtain mRNA loaded lipid nanoparticles;
purifying the mRNA loaded lipid nanoparticle platform using dialysis; and
purifying the mRNA loaded lipid nanoparticle platform using centrifugal filtration.