	US 12,186,429 B2
	Exosome and preparation process and use thereof
Ju-Sheng Shieh, Taipei (TW); and Yu-Tang Chin, Taipei (TW)
Assigned to Ascension Medical Biotechnology Co., Ltd., Taipei (TW)
Filed by Ascension Medical Biotechnology Co., Ltd., Taipei (TW)
Filed on Jun. 24, 2022, as Appl. No. 17/849,389.
Claims priority of application No. 111105325 (TW), filed on Feb. 14, 2022.
Prior Publication US 2023/0255887 A1, Aug. 17, 2023
Int. Cl. A61K 9/127 (2006.01); A61K 8/14 (2006.01); A61P 17/02 (2006.01); A61Q 19/08 (2006.01)

CPC A61K 9/127 (2013.01) [A61K 8/14 (2013.01); A61P 17/02 (2018.01); A61Q 19/08 (2013.01); C12N 2501/999 (2013.01)]

11 Claims

1. A method for producing exosomes, comprising:

cultivating avian embryo-derived mesenchymal stem cells (AMSCs) in a culture medium containing 2,3,4′,5-tetrahydroxystilbene-2-O-β-D-glucoside (THSG) at a concentration ranging from 0.1 μM to 50 μM, so as to obtain a cell culture of AMSCs; and

subjecting the cell culture of AMSCs to a separation treatment, so as to obtain the exosomes.