US 12,186,410 B2
Bi-dota complex-loaded dendritic polymer nanoparticles
Jesus Manuel Perez, Orlando, FL (US); and Santimukul Santra, Orlando, FL (US)
Assigned to University of Central Florida Research Foundation, Inc., Orlando, FL (US)
Filed by UNIVERSITY OF CENTRAL FLORIDA RESEARCH FOUNDATION, INC., Orlando, FL (US)
Filed on Apr. 19, 2021, as Appl. No. 17/234,323.
Application 17/234,323 is a division of application No. 16/317,757, granted, now 10,980,902, previously published as PCT/US2017/042145, filed on Jul. 14, 2017.
Claims priority of provisional application 62/362,323, filed on Jul. 14, 2016.
Prior Publication US 2021/0236665 A1, Aug. 5, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. A61K 51/06 (2006.01); A61K 31/519 (2006.01); A61K 49/04 (2006.01); A61P 35/00 (2006.01); C08G 63/688 (2006.01); C08G 83/00 (2006.01)
CPC A61K 51/065 (2013.01) [A61K 31/519 (2013.01); A61K 49/0423 (2013.01); A61P 35/00 (2018.01); C08G 63/6882 (2013.01); C08G 83/002 (2013.01)] 14 Claims
 
1. A method of identifying a solid tumor cell target, comprising,
1) contacting a cell with an effective amount of a composition comprising at least one polymeric nanoparticle conjugated with a targeting ligand that is a substrate for a solid tumor-specific cell protein, wherein the nanoparticle further comprises an imaging compound and an anti-cancer agent; wherein the polymeric nanoparticle comprises a hyperbranched polyester and/or polyamide polymer, wherein the hyperbranched polyester and/or polyamide polymer has the formula:

OG Complex Work Unit Chemistry
wherein A is a heteroatom independently selected from nitrogen and oxygen; R1 and R2 are independently selected from hydrogen atom, substituted or unsubstituted alkyl, substituted or unsubstituted alkenyl, and substituted or unsubstituted alkynyl; 1 is an integer from 1 to 5; m is 0, 1, or 2; n is an integer from 1 to 5; and o is an integer from 2 or greater;
2) identifying one or more nanoparticles bound to the cells by using imaging devices; and optionally,
3) monitoring the solid tumor cell target by repeating 1) and 2).