	US 12,186,355 B2
	Phytocomplex and selected extract of a meristematic cell line of a plant belonging to the genus melissa
Elena Sgaravatti, Padua (IT); Giovanna Pressi, Rubano (IT); and Flavia Guzzo, Verona (IT)
Assigned to AETHERA BIOTECH S.R.L., (IT)
Appl. No. 17/441,259
Filed by AETHERA BIOTECH S.R.L., Camisano Vicentino (IT)
PCT Filed Mar. 20, 2020, PCT No. PCT/IB2020/052589 § 371(c)(1), (2) Date Sep. 20, 2021, PCT Pub. No. WO2020/188534, PCT Pub. Date Sep. 24, 2020.
Claims priority of application No. 102019000004113 (IT), filed on Mar. 21, 2019.
Prior Publication US 2022/0339232 A1, Oct. 27, 2022
Int. Cl. A61K 36/53 (2006.01); A01H 5/00 (2018.01); A01H 6/50 (2018.01); A61K 8/99 (2017.01); A61Q 19/08 (2006.01)

CPC A61K 36/53 (2013.01) [A01H 5/00 (2013.01); A01H 6/50 (2018.05); A61K 8/99 (2013.01); A61Q 19/08 (2013.01)]

23 Claims

1. A meristematic cell line derived from a plant belonging to the genus Melissa, obtained by means of a process comprising the steps of:

1) plating a tissue obtained from a plant belonging to the genus Melissa onto a solid culture medium;

2) isolating a plurality of cellular clones;

3) inoculating each of the isolated clones into a liquid culture medium;

4) determining the rosmarinic acid and polysaccharide content for each clone;

5) selecting a cellular clone with the highest rosmarinic acid and polysaccharide content, wherein the solid and liquid culture media comprise salts suitable for the growth of plant cells, sucrose, naphthylacetic acid (NAA), indoleacetic acid (IAA) and kinetin, and

wherein said cell line comprises an amount of rosmarinic acid greater than 0.05% w/w, and an amount of polysaccharides from 25% to 70% w/w relative to the dry mass of the cell line.