CPC G01J 3/4406 (2013.01) [G01J 3/0218 (2013.01); G01J 3/0227 (2013.01)] | 20 Claims |
1. A flow cytometry measurement system, comprising:
a flow chamber configured to flow particles of interest in a flow stream;
one or more optical sources configured to excite the particles of interest in the flow stream by an excitation light activated and deactivated according to a pulse train, the particles of interest emitting emission light in response to being excited by the excitation light;
one or more sensor packages each comprising a plurality of photodetectors configured to receive emission light from the particles of interest and, in response, provide i) an output voltage signal corresponding to photoelectron response of an incident photon on the one or more sensor packages, and ii) an output current signal in form of peaks corresponding to photoelectron response of an incident photon on the one or more sensor packages; and
a detector configured to i) determine successive single molecular decay of the particles of interest immediately after the one or more optical sources are deactivated according to the pulse train, ii) generate an emission pulse associated with each incident photon on the one or more sensor packages, and iii) count the number of emission pulses.
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