	US 11,851,719 B2
	Lineage reporter synthetic chromosomes and methods of use
Edward Perkins, Savannah, GA (US); and Amy Greene, Savannah, GA (US)
Assigned to CarryGenes Bioengineering, LLC, Savannah, GA (US)
Filed by SynPloid Biotek, LLC, Savannah, GA (US)
Filed on Sep. 4, 2018, as Appl. No. 16/120,638.
Claims priority of provisional application 62/554,502, filed on Sep. 5, 2017.
Prior Publication US 2019/0071738 A1, Mar. 7, 2019
Int. Cl. C07H 21/04 (2006.01); C12Q 1/6897 (2018.01); C12N 5/0735 (2010.01); C12N 15/85 (2006.01); C12N 5/074 (2010.01); C07K 1/13 (2006.01)

CPC C12Q 1/6897 (2013.01) [C12N 5/0606 (2013.01); C12N 5/0696 (2013.01); C12N 15/85 (2013.01); C07K 1/13 (2013.01); C07K 2319/60 (2013.01); C12N 2510/00 (2013.01); C12N 2800/208 (2013.01); C12N 2800/30 (2013.01); C12N 2830/008 (2013.01)]

1 Claim

1. A non-integrated, transferrable synthetic eukaryotic chromosome comprising:

active centromeric and telomeric regions;

replication origins;

multiple site-specific recombination sequences; and

a plurality of promoters, each promoter operably linked to a different reporter gene, wherein the promoters comprise an OTX2 promoter, a Brachyury promoter, a FLT1 promoter, a GATA4 promoter, and an OCT4 promoter.