CPC C12Q 1/6844 (2013.01) [B01L 3/502 (2013.01); B01L 3/502715 (2013.01); B01L 7/52 (2013.01); C12N 9/1276 (2013.01); C12N 9/22 (2013.01); C12N 9/78 (2013.01); C12Q 1/6806 (2013.01); C12Q 1/701 (2013.01); B01L 2300/0816 (2013.01); C12N 2310/20 (2017.05); C12Q 2600/156 (2013.01); C12Q 2600/16 (2013.01); C12Y 207/07049 (2013.01); G01N 2333/165 (2013.01)] | 14 Claims |
1. A cartridge comprising at least a first and second ampoule, a lysis chamber, an amplification chamber, and a sample receiving chamber, wherein the first ampoule comprises DNA isolation solution capable of isolating a target RNA from a crude or unprocessed sample comprising a cell or virus containing the target RNA and the second ampoule comprises a loop-mediated isothermal amplification (LAMP) composition for detecting the presence of the target RNA in a sample, comprising optimized LAMP primers consisting of SEQ ID NOs: 61983-61988 and amplification reagents for single amplification and glycine or taurine, or a combination thereof for amplifying the target RNA in less than one hour.
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