US 11,851,702 B2
Rapid diagnostics
Feng Zhang, Cambridge, MA (US); Jonathan Gootenberg, Cambridge, MA (US); Omar Abudayyeh, Cambridge, MA (US); Julia Joung, Cambridge, MA (US); Alim Ladha, Cambridge, MA (US); Han Altae-Tran, Cambridge, MA (US); and Guilhem Faure, Cambridge, MA (US)
Assigned to THE BROAD INSTITUTE, INC., Cambridge, MA (US); and MASSACHUSETTS INSTITUTE OF TECHNOLOGY, Cambridge, MA (US)
Filed by THE BROAD INSTITUTE, INC., Cambridge, MA (US); and MASSACHUSETTS INSTITUTE OF TECHNOLOGY, Cambridge, MA (US)
Filed on Jun. 5, 2020, as Appl. No. 16/894,664.
Claims priority of provisional application 62/993,494, filed on Mar. 23, 2020.
Claims priority of provisional application 63/018,487, filed on Apr. 30, 2020.
Claims priority of provisional application 63/019,406, filed on May 3, 2020.
Claims priority of provisional application 63/032,470, filed on May 29, 2020.
Prior Publication US 2021/0292823 A1, Sep. 23, 2021
Int. Cl. C12Q 1/68 (2018.01); C12Q 1/6844 (2018.01); C12N 9/22 (2006.01); C12Q 1/6806 (2018.01); C12N 9/12 (2006.01); C12N 9/78 (2006.01); B01L 3/00 (2006.01); B01L 7/00 (2006.01); C12Q 1/70 (2006.01)
CPC C12Q 1/6844 (2013.01) [B01L 3/502 (2013.01); B01L 3/502715 (2013.01); B01L 7/52 (2013.01); C12N 9/1276 (2013.01); C12N 9/22 (2013.01); C12N 9/78 (2013.01); C12Q 1/6806 (2013.01); C12Q 1/701 (2013.01); B01L 2300/0816 (2013.01); C12N 2310/20 (2017.05); C12Q 2600/156 (2013.01); C12Q 2600/16 (2013.01); C12Y 207/07049 (2013.01); G01N 2333/165 (2013.01)] 14 Claims
OG exemplary drawing
 
1. A cartridge comprising at least a first and second ampoule, a lysis chamber, an amplification chamber, and a sample receiving chamber, wherein the first ampoule comprises DNA isolation solution capable of isolating a target RNA from a crude or unprocessed sample comprising a cell or virus containing the target RNA and the second ampoule comprises a loop-mediated isothermal amplification (LAMP) composition for detecting the presence of the target RNA in a sample, comprising optimized LAMP primers consisting of SEQ ID NOs: 61983-61988 and amplification reagents for single amplification and glycine or taurine, or a combination thereof for amplifying the target RNA in less than one hour.