US 11,851,699 B2
Strand displacement with loop-mediated isothermal amplification
Andrew Ellington, Austin, TX (US); Yu Sherry Jiang, Austin, TX (US); Sanchita Bhadra, Austin, TX (US); Bingling Li, Austin, TX (US); Randy Allen Hughes, Austin, TX (US); Yan Du, Austin, TX (US); and Jimmy Gollihar, Hewitt, TX (US)
Assigned to Board of Regents, The University of Texas System, Austin, TX (US)
Filed by Board of Regents, The University of Texas System, Austin, TX (US)
Filed on Feb. 5, 2021, as Appl. No. 17/168,940.
Application 17/168,940 is a continuation of application No. 14/857,216, filed on Sep. 17, 2015, granted, now 10,913,973.
Claims priority of provisional application 62/051,811, filed on Sep. 17, 2014.
Prior Publication US 2021/0403979 A1, Dec. 30, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/6806 (2018.01); C12Q 1/6897 (2018.01); C12Q 1/6844 (2018.01)
CPC C12Q 1/6806 (2013.01) [C12Q 1/6844 (2013.01); C12Q 1/6897 (2013.01)] 20 Claims
 
1. A method of detecting a nucleic acid, the method comprising
a) amplifying a target nucleic acid using an isothermal amplification reaction, wherein the isothermal amplification reaction produces at least one loop product, wherein at least part of the single-stranded portion of the loop product represents the target nucleic acid;
b) exposing the loop product of step a) to a strand displacement reporter, wherein the strand displacement reporter comprises a single nucleic acid strand comprising a hairpin loop structure and a duplex region, wherein said strand displacement reporter comprises an exposed toehold at a terminus of the strand displacement reporter, and further wherein at least a portion of the duplex region of the strand displacement reporter is complementary to at least a portion of the single-stranded nucleic acid of the loop product representing the target nucleic acid;
c) allowing the loop product and the strand displacement reporter to interact, wherein interaction between the strand displacement reporter and the target nucleic acid portion of the loop product produces a detectable signal, wherein the signal indicates the presence of the target nucleic acid.