CPC C12P 19/34 (2013.01) [C12N 15/11 (2013.01)] | 15 Claims |
1. A method of producing a messenger RNA (mRNA), the method comprising
(a) identifying a non-canonical terminator motif NNATCTGTTNN (SEQ ID NO: 50) in a DNA encoding a mRNA that encodes a polypeptide, wherein N at each position of the non-canonical terminator motif is any nucleotide selected from A, T, C, and G;
(b) producing a modified DNA comprising at least one codon substitution in the non-canonical terminator motif that preserves the amino acid sequence of the polypeptide; and
(c) producing a modified mRNA in an in vitro transcription reaction that comprises the modified DNA, nucleoside triphosphates, and a T7 RNA polymerase.
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