US 11,851,686 B2
Methane monooxygenase enzymes
Elizabeth Jane Clarke, San Francisco, CA (US); Derek Lorin Greenfield, Kensington, CA (US); Noah Charles Helman, El Cerrito, CA (US); Stephanie Rhianon Jones, Berkeley, CA (US); and Baolong Zhu, Johnston, IA (US)
Assigned to Industrial Microbes, Inc., Alameda, CA (US)
Filed by Industrial Microbes, Inc., Alameda, CA (US)
Filed on Aug. 30, 2021, as Appl. No. 17/461,102.
Application 17/461,102 is a continuation of application No. 16/629,018, abandoned, previously published as PCT/US2018/071149, filed on Jul. 6, 2018.
Claims priority of provisional application 62/566,733, filed on Oct. 2, 2017.
Claims priority of provisional application 62/542,838, filed on Aug. 9, 2017.
Claims priority of provisional application 62/529,648, filed on Jul. 7, 2017.
Prior Publication US 2021/0403883 A1, Dec. 30, 2021
Int. Cl. C12N 9/02 (2006.01); C12N 15/70 (2006.01); C12N 15/75 (2006.01); C12N 15/77 (2006.01)
CPC C12N 9/0073 (2013.01) [C12N 15/70 (2013.01); C12N 15/75 (2013.01); C12N 15/77 (2013.01); C12Y 114/13025 (2013.01)] 7 Claims
 
1. A mutant soluble diiron monooxygenase system, comprising a mutant protein folding chaperone groEL-2 comprising an amino acid sequence having at least 80% identity to SEQ ID NO: 9, wherein the mutant protein folding chaperone groEL-2 comprises a mutation at residue position 409, wherein the residue position is numbered with respect to SEQ ID NO: 9.