US 11,851,685 B2
Zwitterion buffer containing compositions and uses in electroanalytical devices and methods
Nathan Allen Carrington, Noblesville, IN (US); Stacy Hunt Duvall, Indianapolis, IN (US); and Leon Scott Van Dyke, Fishers, IN (US)
Assigned to ROCHE DIABETES CARE, INC., Indianapolis, IN (US)
Filed by ROCHE DIABETES CARE, INC., Indianapolis, IN (US)
Filed on Nov. 8, 2021, as Appl. No. 17/520,999.
Application 17/520,999 is a continuation of application No. 16/289,933, filed on Mar. 1, 2019, abandoned.
Application 16/289,933 is a continuation of application No. 13/805,186, abandoned, previously published as PCT/EP2011/003656, filed on Jul. 21, 2011.
Claims priority of provisional application 61/367,243, filed on Jul. 23, 2010.
Prior Publication US 2022/0275348 A1, Sep. 1, 2022
Int. Cl. C12Q 1/00 (2006.01); G01N 27/327 (2006.01); C12N 9/04 (2006.01); C12Q 1/54 (2006.01); C12Q 1/32 (2006.01); C12Q 1/26 (2006.01)
CPC C12N 9/0006 (2013.01) [C12Q 1/006 (2013.01); C12Q 1/26 (2013.01); C12Q 1/32 (2013.01); C12Q 1/54 (2013.01); G01N 27/3271 (2013.01)] 20 Claims
OG exemplary drawing
 
1. An electrochemical sensor comprising an electrode and a dried reagent composition covering at least a portion of the electrode, where the dried reagent composition comprises (a) one or more enzymes selected from the group consisting of glucose dehydrogenases, glucose oxidoreductases, and combinations thereof; (b) one or more mediators, mediator precursors, or a combination thereof, wherein at least one mediator or mediator precursor is aqueous soluble; (c) one or more zwitterionic buffers selected from the group consisting of 2-(N-morpholino)ethanesulfonic acid (MES), piperazine-N,N′-bis(2-ethanesulfonic acid) (PIPES), N-(2-Acetamido)-2-aminoethanesulfonic acid (ACES), (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) (HEPES), N-(Tri(hydroxymethyl)methyl)glycine (tricine), N,N-Bis(2-hydroxyethyl)glycine; (bicine), 3-(N-morpholino)propanesulfonic acid (MOPS), N-cyclohexyl-3-aminopropanesulfonic acid (CAPS), N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic Acid (BES), N-(Hydroxyethyl)piperazine-N′-2-hydroxypropanesulfonic acid (HEPPSO), and N-(2-Acetamido)iminodiacetic acid (ADA); and (d) one or more thickeners; where the sensor is configured for generating a current response to glucose in an aqueous sample; and where the time from contact with the aqueous sample to the DC maximum current response is about 4 seconds or less.