CPC C12N 9/0006 (2013.01) [C12Q 1/006 (2013.01); C12Q 1/26 (2013.01); C12Q 1/32 (2013.01); C12Q 1/54 (2013.01); G01N 27/3271 (2013.01)] | 20 Claims |
1. An electrochemical sensor comprising an electrode and a dried reagent composition covering at least a portion of the electrode, where the dried reagent composition comprises (a) one or more enzymes selected from the group consisting of glucose dehydrogenases, glucose oxidoreductases, and combinations thereof; (b) one or more mediators, mediator precursors, or a combination thereof, wherein at least one mediator or mediator precursor is aqueous soluble; (c) one or more zwitterionic buffers selected from the group consisting of 2-(N-morpholino)ethanesulfonic acid (MES), piperazine-N,N′-bis(2-ethanesulfonic acid) (PIPES), N-(2-Acetamido)-2-aminoethanesulfonic acid (ACES), (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) (HEPES), N-(Tri(hydroxymethyl)methyl)glycine (tricine), N,N-Bis(2-hydroxyethyl)glycine; (bicine), 3-(N-morpholino)propanesulfonic acid (MOPS), N-cyclohexyl-3-aminopropanesulfonic acid (CAPS), N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic Acid (BES), N-(Hydroxyethyl)piperazine-N′-2-hydroxypropanesulfonic acid (HEPPSO), and N-(2-Acetamido)iminodiacetic acid (ADA); and (d) one or more thickeners; where the sensor is configured for generating a current response to glucose in an aqueous sample; and where the time from contact with the aqueous sample to the DC maximum current response is about 4 seconds or less.
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