CPC C12N 5/0636 (2013.01) [A61K 35/17 (2013.01); C12N 7/00 (2013.01); C12N 15/86 (2013.01); G01N 33/56972 (2013.01); C12N 2502/1114 (2013.01); C12N 2740/13043 (2013.01); C12N 2740/15043 (2013.01); G01N 2333/70514 (2013.01); G01N 2333/70517 (2013.01)] | 38 Claims |
1. A method for generating a cell composition, the method comprising:
(a) combining a first cell composition comprising naïve-like CD4+ T cells with a second cell composition comprising naïve-like CD8+ T cells to produce an input cell composition in which the ratio of the naïve-like CD4+ T cells to the naïve-like CD8+ T cells in the input composition is between or about between 0.8:1 and 2.2:1, inclusive;
wherein the ratio has been determined based on the number, number per volume, number per weight, or percentage of naïve-like CD4+ T cells in the first cell composition and the number, number per volume, number per weight, or percentage of naïve-like CD8+ T cells in the second cell composition,
wherein the naïve-like CD4+ cells:
(i) are surface positive for CD45RA and CCR7;
(ii) are surface positive CD27 and CCR7; or
(iii) are surface positive for CCR7 and surface negative for CD62L; and
wherein the naïve-like CD8+ cells:
(i) are surface positive for CD45RA and CCR7;
(ii) are surface positive CD27 and CCR7; or
(iii) are surface positive for CCR7 and surface negative for CD62L; and
(b) contacting the input cell composition with an agent comprising a nucleic acid molecule encoding a recombinant receptor under conditions to introduce the nucleic acid molecule encoding the recombinant receptor into the input cell composition.
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