US 11,851,644 B2
Marine bacteria formulation useful in aquaculture
David R. Nelson, Wakefield, RI (US); Jason LaPorte, Riverside, RI (US); David C Rowley, Wakefield, RI (US); and Marta Gomez-Chiarri, Kingston, RI (US)
Assigned to University of Rhode Island Board of Trustees, Kingston, RI (US)
Filed by University of Rhode Island Board of Trustees, Kingston, RI (US)
Filed on May 4, 2020, as Appl. No. 16/866,265.
Claims priority of provisional application 62/842,282, filed on May 2, 2019.
Prior Publication US 2020/0347345 A1, Nov. 5, 2020
Int. Cl. C12N 1/20 (2006.01); A61K 35/741 (2015.01)
CPC C12N 1/20 (2013.01) [A61K 35/741 (2013.01); C12N 2500/05 (2013.01); C12N 2500/32 (2013.01); C12N 2500/74 (2013.01)] 20 Claims
 
1. A method of making a heterotrophic and probiotic marine bacterial composition suitable for long-term storage and transportation, comprising:
(a) growing a population of heterotrophic and probiotic marine bacteria to a high density of about 1×106 CFU/ml or higher in a broth medium containing at least one ingredient selected from the group consisting of peptone, tryptone, and yeast extract; subsequently
(b) adding to the bacteria-bearing broth medium both (i) water between (1:1) and (1:2) (medium:water) by volume to dilute the medium to lower ingredient concentrations that sustain long-term bacterial vitality wherein any peptone or tryptone is diluted to at least about 1.7 g/L but no more than about 7.5 g/L and any yeast extract is diluted to at least about 0.3 g/L but no more than about 3.0 g/L in concentrations, and (ii) a sufficient amount of salt to maintain a total salt concentration at or above 2.0%, wherein further bacterial growth is slowed down but bacterial vitality is preserved and, after six weeks, at least about 50% of the bacteria population remain viable; and
(c) storing and/or transporting the broth medium from step (b) wherein at least about 50% of the heterotrophic and probiotic marine bacteria composition contained therein remained viable.