CPC C12N 1/20 (2013.01) [A61K 35/741 (2013.01); C12N 2500/05 (2013.01); C12N 2500/32 (2013.01); C12N 2500/74 (2013.01)] | 20 Claims |
1. A method of making a heterotrophic and probiotic marine bacterial composition suitable for long-term storage and transportation, comprising:
(a) growing a population of heterotrophic and probiotic marine bacteria to a high density of about 1×106 CFU/ml or higher in a broth medium containing at least one ingredient selected from the group consisting of peptone, tryptone, and yeast extract; subsequently
(b) adding to the bacteria-bearing broth medium both (i) water between (1:1) and (1:2) (medium:water) by volume to dilute the medium to lower ingredient concentrations that sustain long-term bacterial vitality wherein any peptone or tryptone is diluted to at least about 1.7 g/L but no more than about 7.5 g/L and any yeast extract is diluted to at least about 0.3 g/L but no more than about 3.0 g/L in concentrations, and (ii) a sufficient amount of salt to maintain a total salt concentration at or above 2.0%, wherein further bacterial growth is slowed down but bacterial vitality is preserved and, after six weeks, at least about 50% of the bacteria population remain viable; and
(c) storing and/or transporting the broth medium from step (b) wherein at least about 50% of the heterotrophic and probiotic marine bacteria composition contained therein remained viable.
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