US 11,851,641 B2
Method for producing stem cell culture plate available for tissue engineering using 3D printing for human organoid generation
Dawitt Kang, Seoul (KR); Hyunchul Kang, Bucheon-si (KR); Jungin Yoon, Hwaseong-si (KR); Chaeyeon Kim, Jeollanam-do (KR); and Jeongwon Rho, Seoul (KR)
Assigned to HAN BIO USA; and HANBIO CO., LTD., Seoul (KR)
Filed by HAN BIO USA, Los Angeles, CA (US); and HANBIO CO., LTD., Seoul (KR)
Filed on Jul. 8, 2020, as Appl. No. 16/923,868.
Claims priority of provisional application 62/894,483, filed on Aug. 30, 2019.
Prior Publication US 2021/0062125 A1, Mar. 4, 2021
Int. Cl. B33Y 10/00 (2015.01); C12M 3/00 (2006.01); C12N 5/077 (2010.01); B33Y 80/00 (2015.01); B29C 64/118 (2017.01); B33Y 70/00 (2020.01); B29K 67/00 (2006.01)
CPC C12M 21/08 (2013.01) [B29C 64/118 (2017.08); B33Y 10/00 (2014.12); B33Y 70/00 (2014.12); B33Y 80/00 (2014.12); C12N 5/0653 (2013.01); C12N 5/0654 (2013.01); C12N 5/0658 (2013.01); B29K 2067/046 (2013.01); B29K 2995/006 (2013.01); C12N 2506/1384 (2013.01); C12N 2513/00 (2013.01)] 3 Claims
OG exemplary drawing
 
1. A method for producing a cell culture plate for human organoid generation, the method comprising the steps of:
(a) fabricating a film by dissolving a mixture of polyactic acid (PLA) and polycaprolactone (PCL) in a volume equal to 1/10 of Dichloromethane (DCM) and adding a compatibilizer for improving interfacial adhesion to the mixture in an amount of about 3 wt % of the PCL;
(b) fabricating a fillet after drying the film at room temperature around 24 hours;
(c) fabricating a filament of the fillet with a filament extruder; and
(d) producing the cell culture plate using a 3D printer after feeding the filament into the 3D printer.