US 11,851,488 B2
Anti-CD3-folate conjugates and their uses
Harun Rashid, San Diego, CA (US); Feng Tian, San Diego, CA (US); and Marco Gymnopoulos, San Diego, CA (US)
Assigned to Ambrx, Inc., La Jolla, CA (US)
Appl. No. 15/773,167
Filed by Ambrx, Inc., La Jolla, CA (US)
PCT Filed Nov. 2, 2016, PCT No. PCT/US2016/060087
§ 371(c)(1), (2) Date May 3, 2018,
PCT Pub. No. WO2017/079272, PCT Pub. Date May 11, 2017.
Claims priority of provisional application 62/374,693, filed on Aug. 12, 2016.
Claims priority of provisional application 62/267,086, filed on Dec. 14, 2015.
Claims priority of provisional application 62/250,451, filed on Nov. 3, 2015.
Prior Publication US 2018/0319885 A1, Nov. 8, 2018
Int. Cl. C07K 16/28 (2006.01); A61K 47/68 (2017.01); A61K 39/395 (2006.01); A61K 31/522 (2006.01); A61K 39/00 (2006.01)
CPC C07K 16/2809 (2013.01) [A61K 31/522 (2013.01); A61K 39/395 (2013.01); A61K 47/6803 (2017.08); A61K 47/6849 (2017.08); A61K 2039/505 (2013.01); C07K 2317/55 (2013.01); C07K 2317/622 (2013.01); C07K 2317/92 (2013.01); C07K 2317/94 (2013.01)] 23 Claims
 
1. An anti-human CD3 antibody, comprising (a) a heavy chain amino acid sequence comprising a sequence selected from the group consisting of SEQ ID NOs: 1, 2 and 3, (b) a light chain amino acid sequence comprising a sequence selected from the group consisting of SEQ ID NOs: 4, 5 and 6, and (c) a non-naturally encoded amino acid substituted for position 114Ala of the heavy chain, position 115Ser of the heavy chain, or position 129Lys of the heavy chain, wherein said non-naturally encoded amino acid is para-acetyl-phenylalanine, para-azido-phenylalanine or p-propargyloxyphenylalanine.