CPC C07D 257/02 (2013.01) [C07C 233/44 (2013.01); C07C 309/65 (2013.01); C07D 213/38 (2013.01); G01N 21/64 (2013.01)] | 11 Claims |
1. A method of detecting negatively charged phosphate-containing membrane components comprising:
(a) combining a solution of a sample suspected of comprising negatively charged phosphate-containing membrane components with a solution comprising a compound of Formula V, or a metal chelate salt thereof:
wherein
L2 is a linker group selected from C1-20alkylene, C2-20alkenylene, C2-20alkynylene and C3-10-cycloalkylene, or a combination thereof, each of which is optionally oxo-substituted (═O) 1-3 times, and in which 1-3 carbon atoms are optionally replaced with a heteromoiety selected from NH, O, S and Si; and
R3 is selected from
(b) detecting a fluorescence signal;
wherein detection of the fluorescence signal in (b) indicates that the sample comprises negatively charged phosphate-containing membrane components.
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