US 11,850,535 B2
Methods for quantitating individual antibodies from a mixture
Dingjiang Liu, Pleasantville, NY (US); Lin Luo, Paramus, NJ (US); and Long Xu, Shanghai (CN)
Assigned to Regeneron Pharmaceuticals, Inc., Tarrytown, NY (US)
Filed by Regeneron Pharmaceuticals, Inc., Tarrytown, NY (US)
Filed on Dec. 19, 2022, as Appl. No. 18/067,803.
Application 18/067,803 is a continuation of application No. 17/321,663, filed on May 17, 2021, granted, now 11,571,636.
Application 17/321,663 is a continuation of application No. 16/322,292, granted, now 11,020,686, issued on Jun. 1, 2021, previously published as PCT/US2017/045855, filed on Aug. 8, 2017.
Claims priority of provisional application 62/375,887, filed on Aug. 16, 2016.
Prior Publication US 2023/0119592 A1, Apr. 20, 2023
Int. Cl. B01D 15/30 (2006.01); G01N 33/68 (2006.01); B01D 15/32 (2006.01); A61K 39/395 (2006.01); C07K 16/00 (2006.01); C07K 16/06 (2006.01); C07K 16/10 (2006.01); G01N 30/02 (2006.01)
CPC B01D 15/30 (2013.01) [A61K 39/39591 (2013.01); B01D 15/327 (2013.01); C07K 16/00 (2013.01); C07K 16/06 (2013.01); C07K 16/065 (2013.01); C07K 16/10 (2013.01); G01N 30/02 (2013.01); G01N 33/6854 (2013.01); G01N 2030/027 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A method of quantitating antibodies in a mixture comprising a plurality of antibodies, the method comprising:
generating a standard curve for each antibody of the plurality of antibodies;
separating each antibody of the plurality of antibodies by using hydrophobic interaction chromatography high performance liquid chromatography (HIC-HPLC); and
after separating each antibody, quantitating an amount of each antibody using the standard curve;
wherein two or more of the antibodies in the mixture are of the same isotype.