US 11,850,284 B2
Compositions and methods for delivery of nucleic acids to cells
Elias Quijano, Durham, CT (US); and Peter Glazer, Guilford, CT (US)
Assigned to Yale University, New Haven, CT (US)
Filed by Yale University, New Haven, CT (US)
Filed on Aug. 30, 2022, as Appl. No. 17/823,494.
Application 17/823,494 is a continuation of application No. 17/638,642, previously published as PCT/US2020/048823, filed on Aug. 31, 2020.
Application 17/638,642 is a continuation in part of application No. PCT/US2019/048962, filed on Aug. 30, 2019.
Application PCT/US2019/048962 is a continuation in part of application No. PCT/US2019/048953, filed on Aug. 30, 2019.
Claims priority of provisional application 62/944,281, filed on Dec. 5, 2019.
Prior Publication US 2023/0093888 A1, Mar. 30, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. A61K 47/54 (2017.01); A61P 35/00 (2006.01); C07K 16/44 (2006.01); A61K 48/00 (2006.01); A61K 47/68 (2017.01); C07K 14/47 (2006.01); C07K 14/52 (2006.01); A61K 39/00 (2006.01); C12N 15/113 (2010.01); C12N 15/11 (2006.01); C12N 15/87 (2006.01)
CPC A61K 47/549 (2017.08) [A61K 47/6807 (2017.08); A61K 47/6851 (2017.08); A61K 48/005 (2013.01); A61P 35/00 (2018.01); C07K 14/4747 (2013.01); C07K 14/52 (2013.01); C07K 16/44 (2013.01); C12N 15/111 (2013.01); C12N 15/1135 (2013.01); C12N 15/87 (2013.01); A61K 48/00 (2013.01); A61K 48/0025 (2013.01); A61K 2039/505 (2013.01); C07K 2317/24 (2013.01); C07K 2317/31 (2013.01); C07K 2317/565 (2013.01); C07K 2317/622 (2013.01); C07K 2317/77 (2013.01); C07K 2319/33 (2013.01); C12N 15/113 (2013.01); C12N 2310/11 (2013.01); C12N 2310/14 (2013.01); C12N 2310/3181 (2013.01); C12N 2310/3513 (2013.01); C12N 2320/32 (2013.01)] 22 Claims
 
1. A composition comprising a non-covalent complex of (a) an antibody or antigen-binding fragment thereof, and (b) an mRNA,
wherein the antibody or antigen-binding fragment thereof comprises heavy chain variable region (VH) complementarity determining regions (CDRs) having the amino acid sequences of SEQ ID NO:16, SEQ ID NO:17, and SEQ ID NO:18, and light chain variable region (VL) CDRs having the amino acid sequences of SEQ ID NO:24, SEQ ID NO:25, and SEQ ID NO:26.