US 11,850,216 B2
18F labeling of proteins using sortases
Mohammad Rashidian, Cambridge, MA (US); Hidde L. Ploegh, Boston, MA (US); Ralph Weissleder, Peabody, MA (US); and Edmund J. Keliher, Topsfield, MA (US)
Assigned to Whitehead Institute for Biomedical Research, Cambridge, MA (US); and The General Hospital Corporation, Boston, MA (US)
Filed by Whitehead Institute for Biomedical Research, Cambridge, MA (US); and The General Hospital Corporation, Boston, MA (US)
Filed on Jan. 8, 2020, as Appl. No. 16/737,620.
Application 16/737,620 is a division of application No. 15/035,924, granted, now 10,556,024, previously published as PCT/US2014/065574, filed on Nov. 13, 2014.
Claims priority of provisional application 61/903,834, filed on Nov. 13, 2013.
Prior Publication US 2020/0384137 A1, Dec. 10, 2020
Int. Cl. A61K 51/10 (2006.01); A61K 51/08 (2006.01); G01N 33/60 (2006.01); C12N 9/52 (2006.01); C07H 5/02 (2006.01); C07K 16/28 (2006.01)
CPC A61K 51/1096 (2013.01) [A61K 51/08 (2013.01); C07H 5/02 (2013.01); C07K 16/2845 (2013.01); C12N 9/52 (2013.01); C12Y 304/2207 (2013.01); G01N 33/60 (2013.01); C07K 2317/55 (2013.01); C07K 2317/569 (2013.01); G01N 2333/95 (2013.01); G01N 2800/52 (2013.01); G01N 2800/7028 (2013.01)] 21 Claims
 
1. A radioactive protein of Formula (II):

OG Complex Work Unit Chemistry
wherein
custom character represents a protein;
L1 is a peptide linker comprising -LPXTGGGK-, -LPXTGGG-, -NPXTGGGK-, -NPXTGGG-, -LPXTAAA-, -NPXTAAA-, -LPXTGGGGG-, or -LPGAG-, wherein each instance of X is independently any amino acid; and
L2 is a linker comprising optionally substituted heterocyclylene.