US 11,849,709 B2
Genetically modified rat models for severe combined immunodeficiency (SCID)
Eric M. Ostertag, Del Mar, CA (US); John Stuart Crawford, Lexington, KY (US); and Joseph Ruiz, Cary, NC (US)
Assigned to Hera Testing Laboratories, Inc., Lexington, KY (US)
Filed by Hera Testing Laboratories, Inc., Lexington, KY (US)
Filed on Dec. 16, 2021, as Appl. No. 17/553,463.
Application 17/553,463 is a continuation of application No. 17/335,568, filed on Jun. 1, 2021.
Application 17/335,568 is a continuation of application No. 15/810,272, filed on Nov. 13, 2017, granted, now 11,089,765, issued on Aug. 17, 2021.
Application 15/810,272 is a continuation of application No. 15/070,275, filed on Mar. 15, 2016, abandoned.
Application 15/070,275 is a continuation of application No. 13/391,307, granted, now 9,314,005, issued on Apr. 19, 2016, previously published as PCT/US2010/040768, filed on Jul. 1, 2010.
Claims priority of provisional application 61/222,327, filed on Jul. 1, 2009.
Prior Publication US 2022/0167598 A1, Jun. 2, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. A01K 67/027 (2006.01); C12N 9/12 (2006.01); C12N 9/14 (2006.01); C12N 9/22 (2006.01); C12N 15/85 (2006.01); C12N 15/87 (2006.01); C12N 15/90 (2006.01)
CPC A01K 67/0276 (2013.01) [C12N 9/1205 (2013.01); C12N 9/14 (2013.01); C12N 9/22 (2013.01); C12N 15/8509 (2013.01); C12N 15/87 (2013.01); C12N 15/907 (2013.01); A01K 2217/05 (2013.01); A01K 2217/054 (2013.01); A01K 2217/056 (2013.01); A01K 2217/20 (2013.01); A01K 2217/206 (2013.01); A01K 2227/105 (2013.01); A01K 2267/0331 (2013.01); A01K 2267/0387 (2013.01); C12N 2015/8536 (2013.01)] 21 Claims
 
1. A genetically engineered rat, or progeny of the rat, whose genome comprises a homozygous knock-out of the interleukin 2 receptor subunit gamma (Il2rg) gene and a homozygous knock-out of the recombination activating gene 1 (Rag 1) gene, wherein the rat or its progeny exhibits Severe Combined Immunodeficiency (SCID), wherein the rat or its progeny has decreased levels of mature T-cells, B-cells and NK-cells, as compared to a rat not comprising the knock-out of the Il2rg gene and the knock-out of the Rag1 gene.