US 10,179,938 C2 (12,472nd)
Artificial selection method and reagents
Ben Hayes, Kensington (AU); and Michael Goddard, Diamond Creek (AU)
Filed by Agriculture Victoria Services Pty Limited, Attwood (AU)
Reexamination Request No. 90/014,582, Sep. 21, 2020.
Reexamination Certificate for Patent 10,179,938, issued Jan. 15, 2019, Appl. No. 14/245,334, Apr. 4, 2014.
Reexamination Certificate C1 10,179,938, issued Dec. 28, 2021.
Application 90/014,582 is a continuation of application No. 12/448,463, abandoned, previously published as PCT/AU2007/002006, filed on Dec. 21, 2007.
Claims priority of provisional application 60/876,623, filed on Dec. 21, 2006.
Ex Parte Reexamination Certificate issued on Dec. 18, 2023.
Int. Cl. C12Q 1/68 (2018.01); C12Q 1/6888 (2018.01); A01K 67/02 (2006.01)
CPC C12Q 1/6888 (2013.01) [A01K 67/02 (2013.01); C12Q 1/68 (2013.01)]
AS A RESULT OF REEXAMINATION, IT HAS BEEN DETERMINED THAT:
Claims 1-16 are cancelled.
1. A method of selective breeding in a non-human population having a genetic diversity equivalent to an effective population size (Ne) of less than 1000, said method comprising:
(i) detecting, in nucleic acids isolated from more than one chromosome segment of a plurality of individuals in the non-human population, the presence or absence of more than one set of informative markers, wherein each set of informative markers is a set of informative markers at a gene or locus associated with a heritable trait on a chromosome segment, to thereby determine the genotype of each individual at each set of such informative markers, and wherein each set of informative markers is a set of known informative markers and wherein for each set of informative markers, the genotype at one or more other known informative markers at the gene or locus associated with the heritable trait is not determined;
(ii) for each individual, comparing the genotype at each set of informative markers determined in step (i) to the genotype at each such set of informative markers in an ancestor and/or founder contributing at least 0.1% of the total genetic variance to the current population to thereby determine the lineage of each of the chromosome segments of the individual from an ancestor and/or founder of the population from which each of the chromosome segments are derived, wherein the genotype of the ancestor and/or founder is known for each of the more than one set of informative markers detected in each individual of step (i) as well as for at least one of the one or more other known informative markers of each set for which the genotype(s) was/were not determined for each individual in step (i);
(iii) for each individual, inferring that the genotype at the one or more other known informative markers of each set for which the genotype(s) was/were not determined in step (i) is the same as for the ancestor and/or founder based on the lineage of the chromosome segments determined at (ii) to thereby produce one or more inferred genotype(s) for each set of informative markers;
(iv) estimating the breeding value of each individual based on genotypes comprising the one or more inferred genotype(s) determined in step (iii) to produce an estimated breeding value (EBV) for each said individual;
(v) selecting from the plurality of individuals, an individual having a high EBV;
(vi) obtaining reproductive or regenerative material from the individual selected, and
(vii) producing one or more individuals or one or more generations of individuals from the reproductive or regenerative material, thereby selectively breeding in the non-human population.