US 11,835,526 B2
Functionalized chromophoric polymer dots and bioconjugates thereof
Daniel T. Chiu, Seattle, WA (US); Changfeng Wu, Changchun (CN); Jason McNeill, Clemson, SC (US); and Jiangbo Yu, Bothell, WA (US)
Assigned to University of Washington, Seattle, WA (US); and Clemson University Research Foundation, Clemson, SC (US)
Filed by University of Washington, Seattle, WA (US); and Clemson University Research Foundation, Clemson, SC (US)
Filed on Feb. 15, 2022, as Appl. No. 17/672,586.
Application 17/672,586 is a continuation of application No. 16/209,729, filed on Dec. 4, 2018, granted, now 11,249,086, issued on Feb. 15, 2022.
Application 16/209,729 is a continuation of application No. 13/508,981, granted, now 10,191,060, previously published as PCT/US2010/056079, filed on Nov. 9, 2010.
Claims priority of provisional application 61/259,611, filed on Nov. 9, 2009.
Prior Publication US 2022/0252607 A1, Aug. 11, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 33/58 (2006.01); C09K 11/06 (2006.01)
CPC G01N 33/587 (2013.01) [C09K 11/06 (2013.01); G01N 33/582 (2013.01)] 18 Claims
 
1. A method for detecting biological molecules, the method comprising:
adhering a chromophoric polymer dot to a biological molecule associated with a cell in a fluid, thereby forming a bioconjugated chromophoric polymer dot, wherein the chromophoric polymer dot comprises a chromophoric polymer and an amphiphilic molecule, wherein:
the amphiphilic molecule comprises a hydrophobic moiety and a hydrophilic moiety;
the hydrophilic moiety comprises one or more reactive functional groups;
the weight ratio of the amphiphilic molecule to the chromophoric polymer is from about 1% to about 50%; and
detecting the bioconjugated chromophoric polymer dot using one or more of flow cytometry or fluorescence microscopy imaging.