CPC G01N 33/587 (2013.01) [C09K 11/06 (2013.01); G01N 33/582 (2013.01)] | 18 Claims |
1. A method for detecting biological molecules, the method comprising:
adhering a chromophoric polymer dot to a biological molecule associated with a cell in a fluid, thereby forming a bioconjugated chromophoric polymer dot, wherein the chromophoric polymer dot comprises a chromophoric polymer and an amphiphilic molecule, wherein:
the amphiphilic molecule comprises a hydrophobic moiety and a hydrophilic moiety;
the hydrophilic moiety comprises one or more reactive functional groups;
the weight ratio of the amphiphilic molecule to the chromophoric polymer is from about 1% to about 50%; and
detecting the bioconjugated chromophoric polymer dot using one or more of flow cytometry or fluorescence microscopy imaging.
|