US 11,835,525 B2
Multi-spectral microparticle-fluorescence photon cytometry
Masanobu Yamamoto, West Lafayette, IN (US); and J. Paul Robinson, West Lafayette, IN (US)
Assigned to Miftek Corporation, West Lafayette, IN (US)
Filed by MIFTEK CORPORATION, West Lafayette, IN (US)
Filed on Apr. 3, 2020, as Appl. No. 16/840,220.
Application 16/840,220 is a continuation of application No. 15/250,136, filed on Aug. 29, 2016, granted, now 10,613,096.
Claims priority of provisional application 62/211,452, filed on Aug. 28, 2015.
Prior Publication US 2020/0340999 A1, Oct. 29, 2020
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 33/58 (2006.01); B01L 3/00 (2006.01); G01N 35/00 (2006.01); G01N 15/14 (2006.01); G01N 33/49 (2006.01); G01N 21/64 (2006.01)
CPC G01N 33/582 (2013.01) [B01L 3/00 (2013.01); G01N 15/1427 (2013.01); G01N 15/1429 (2013.01); G01N 15/1434 (2013.01); G01N 33/4915 (2013.01); G01N 35/00069 (2013.01); G01N 15/1456 (2013.01); G01N 15/1459 (2013.01); G01N 15/1484 (2013.01); G01N 21/645 (2013.01); G01N 2035/00881 (2013.01); G01N 2201/1045 (2013.01)] 8 Claims
OG exemplary drawing
 
1. A flow cytometer for observing a microparticulate target, the flow cytometer comprising:
a flow chamber including a flow channel formed therein to permit the microparticulate target to flow in a flow direction through a sensing region of the flow channel;
an irradiation optical system configured to irradiate the microparticulate target in the sensing region with incident light;
an optical disperser configured to spectrally disperse at least a first portion of resultant light originating from within the sensing region to provide multiple light beams associated with respective, different wavelengths;
multiple photodetectors configured to receive respective light beams of the multiple light beams and provide respective resultant-light signals; and
a spot-traversal system for causing motion of an irradiation spot substantially across the microparticulate target in the sensing region,
wherein the irradiation optical system is configured to irradiate the microparticulate target with the incident light configured as an irradiation spot.