CPC G01N 33/526 (2013.01) [G01N 21/77 (2013.01); G01N 2021/7759 (2013.01)] | 18 Claims |
1. A method of evaluating suitability of lighting conditions for detecting an analyte in a sample using a mobile device having a camera, the method comprising:
a) providing a test strip configured for detecting the analyte in the sample and having at least one test field with a test chemical for an optical detection reaction;
b) capturing a first image of the test strip while an illumination source of the mobile device is turned off;
c) capturing a second image of the test strip while the illumination source is turned on, wherein the illumination source generates illumination having a known or predetermined spectral composition;
d) applying a sample to the test strip prior to step c);
e) comparing the first and second images and thereby determining a difference in lighting conditions between the first image and the second image, wherein the camera captures the images in steps b) and c) in at least one color channel and the camera and/or a processor of the mobile device determines first and second intensity distributions, respectively, for the at least one color channel associated with the first and second images, wherein the comparing the first and second images comprises comparing the first intensity distribution and the second intensity distribution of the at least one color channel;
f) determining an intensity of the illumination which originates from the illumination source used for illumination of the test strip when capturing the second image as a function of the known or predetermined spectral composition and wherein the known or predetermined spectral composition of the illumination source is obtained before capturing an image of the test strip of step a) with the mobile device;
g) deriving suitability information of the lighting conditions for analyte detection from the comparison in step e) and the determination of step f); and
h) determining that the lighting conditions are suitable for subsequent analyte detection using the second image when at least 70% of light intensity used for illumination of the test strip in the second image originates from the illumination source.
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