CPC G01N 21/6458 (2013.01) [G01J 3/28 (2013.01); G01J 3/2823 (2013.01); G01N 21/6428 (2013.01); G06T 7/0012 (2013.01); G06T 7/194 (2017.01); G01J 2003/2826 (2013.01); G01N 2021/6419 (2013.01); G01N 2021/6421 (2013.01); G06T 2207/10036 (2013.01); G06T 2207/10056 (2013.01); G06T 2207/10064 (2013.01); G06T 2207/30024 (2013.01)] | 24 Claims |
1. A method, comprising:
obtaining one or more images of a biological sample, wherein each sample image corresponds to a different combination of illumination light wavelengths and light emission wavelengths; and
obtaining a background image of the biological sample by exposing the biological sample to background excitation light and measuring light emission from the biological sample at one or more background emission wavelengths that are each within a wavelength range defined by the light emission wavelengths,
wherein for each of one or more spectral contributors in the biological sample exposed to the background excitation light, a spectral emission intensity at each of the background emission wavelengths is 10% or less of a maximum spectral emission intensity of the spectral contributor following excitation at each of the illumination light wavelengths; and
wherein a distribution of the background emission wavelengths has a full width at half maximum (FWHM) spectral width Δλ and center wavelength λc, and the background image corresponds to measured light emission for at least some wavelengths within a range from λc-λ/2 to λc+λ/2.
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