US 11,835,461 B2
Microscopy system and method for operating a microscopy system
Marco Wilzbach, Aalen (DE); Christoph Nieten, Jena (DE); and Stefan Meinkuss, Aalen (DE)
Assigned to Carl Zeiss Meditec AG, Jena (DE)
Filed by Carl Zeiss Meditec AG, Jena (DE)
Filed on Jan. 24, 2020, as Appl. No. 16/752,610.
Claims priority of application No. 10 2019 101 773.4 (DE), filed on Jan. 24, 2019.
Prior Publication US 2020/0240918 A1, Jul. 30, 2020
Int. Cl. G01N 21/64 (2006.01); G02B 21/00 (2006.01); G02B 21/36 (2006.01)
CPC G01N 21/6458 (2013.01) [G02B 21/0076 (2013.01); G02B 21/365 (2013.01)] 43 Claims
OG exemplary drawing
 
1. A microscopy system for simultaneously observing fluorescent and non-fluorescent regions of an object, the microscopy system comprising:
a microscopy optical unit configured to image an object plane through an observation beam path onto an image plane;
an observation filter arrangeable in the observation beam path, a transmittance of the observation filter from λ1 to λ2 being less than W1, from λ3 to λ4 being larger than W2, and from λ5 to λ6 being larger than W3, λ1, λ2, λ3, λ4, λ5, and λ6 being wavelengths for which 350 nm <λ1<λ2≤λ3<λ4≤λ5<λ6<750 nm holds true, and W1, W2, and W3 being values for which 0<W1<W2<W3<1 holds true;
a first light source configured to generate first light having wavelengths of between λ1 and λ2 and to direct the first light onto the object plane;
a second light source configured to generate second light having wavelengths of between λ3 and λ4 and to direct the second light onto the object plane; and
a controller configured to control the first light source and/or the second light source individually, as a result of which a ratio of the intensity of the first light to the intensity of the second light is variable.