CPC C12Q 1/6886 (2013.01) [C12Q 1/6869 (2013.01); G16B 30/00 (2019.02); G16B 30/10 (2019.02); C12Q 2600/106 (2013.01); C12Q 2600/118 (2013.01); C12Q 2600/154 (2013.01); C12Q 2600/156 (2013.01)] | 10 Claims |
1. A method of treating cancer in a subject comprising calculating a proportion of discordant reads (PDR) in a tumor sample from the subject comprising:
detecting DNA methylation status by massive parallel sequencing with bisulfite conversion; whole-genome bisulfite sequencing; reduced representation bisulfite sequencing; microarray, or a genome-wide microarray at one or more regions of neighboring CpG sites in a plurality of cells in the tumor sample;
comparing the DNA methylation status in multiple regions of neighboring CpG sites along a sequence of CpG sites in DNA of the plurality of cells; and
determining a relative number of cells in the tumor sample having inconsistent DNA methylation status across the sequence of CpG sites as compared to the total number of cells in the tumor sample or a number of cells in the tumor sample having consistent DNA methylation status across the sequence of CpG sites thereby calculating the PDR in the tumor sample,
determining the PDR in the tumor sample to be less than or equal to 0.15, and
administering to the subject an antitumor agent.
|