US 11,834,703 B2
Hybridization compositions and methods
Charles M. Hansen, Horsholm (DK); Steen Hauge Matthiesen, Hillerød (DK); Kenneth H. Petersen, Smørum (DK); and Tim Svenstrup Poulsen, Horsholm (DK)
Assigned to Agilent Technologies, Inc., Santa Clara, CA (US)
Filed by Agilent Technologies, Inc., Santa Clara, CA (US)
Filed on Sep. 13, 2021, as Appl. No. 17/473,524.
Application 17/473,524 is a continuation of application No. 12/994,492, granted, now 11,118,214, previously published as PCT/IB2009/005893, filed on May 27, 2009.
Claims priority of provisional application 61/155,683, filed on Feb. 26, 2009.
Claims priority of provisional application 61/056,089, filed on May 27, 2008.
Claims priority of application No. PA200800727 (DK), filed on May 27, 2008; and application No. PA200900278 (DK), filed on Feb. 27, 2009.
Prior Publication US 2022/0064716 A1, Mar. 3, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/6832 (2018.01); C12Q 1/6841 (2018.01)
CPC C12Q 1/6832 (2013.01) [C12Q 1/6841 (2013.01); Y10T 436/143333 (2015.01)] 19 Claims
 
1. A hybridization composition comprising a mixture of at least one nucleic acid sequence, at least one polar aprotic solvent in an amount effective to denature double-stranded nucleotide sequences, an accelerating agent, and at least one additional component selected from the group consisting of: buffering agents, salts, chelating agents, detergents, and blocking agents,
wherein less than 10% of the hybridization composition is formamide,
wherein the polar aprotic solvent is not dimethyl sulfoxide (DMSO) and has lactone, sulfone, sulfite, and/or carbonate functional groups,
wherein the at least one nucleic acid sequence is a FISH or CISH probe;
wherein the at least one polar aprotic solvent comprises a polar aprotic solvent selected from the group consisting of:

OG Complex Work Unit Chemistry
and
wherein the accelerating agent is a polymer.