CPC C12Q 1/6832 (2013.01) [C12Q 1/6841 (2013.01); Y10T 436/143333 (2015.01)] | 19 Claims |
1. A hybridization composition comprising a mixture of at least one nucleic acid sequence, at least one polar aprotic solvent in an amount effective to denature double-stranded nucleotide sequences, an accelerating agent, and at least one additional component selected from the group consisting of: buffering agents, salts, chelating agents, detergents, and blocking agents,
wherein less than 10% of the hybridization composition is formamide,
wherein the polar aprotic solvent is not dimethyl sulfoxide (DMSO) and has lactone, sulfone, sulfite, and/or carbonate functional groups,
wherein the at least one nucleic acid sequence is a FISH or CISH probe;
wherein the at least one polar aprotic solvent comprises a polar aprotic solvent selected from the group consisting of:
and
wherein the accelerating agent is a polymer.
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