CPC C12N 5/0693 (2013.01) [C12N 2500/02 (2013.01); C12N 2500/90 (2013.01); C12N 2501/105 (2013.01); C12N 2501/115 (2013.01); C12N 2501/415 (2013.01); C12N 2501/998 (2013.01)] | 12 Claims |
1. A method for producing an organoid comprising:
preparing an extracellular matrix,
adhering epithelial stem cells, epithelial cells, epithelial tumor cells, or tissue at least containing any one of these cells onto the extracellular matrix, and
forming the organoid by adding a cell culture medium for culturing the organoid and culturing the epithelial stem cells, the epithelial cells, the epithelial tumor cells, or tissue at least containing any one of these cells, after adhering,
wherein the cell culture medium for culturing the organoid comprises at least two types of components selected from the group consisting of insulin-like growth factor 1 (IGF1), fibroblast growth factor 2 (FGF2) and epiregulin (EREG), and at least one type of component among the following components i) to iii):
i) Wnt agonist,
ii) bone morphogenetic protein (BMP) inhibitor, and
iii) transforming growth factor-β (TGF-β) inhibitor,
wherein the cell culture medium for culturing the organoid does not substantially contain EGF and p38 inhibitor.
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